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Registros recuperados : 170 | |
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Registro Completo
Biblioteca(s): |
Embrapa Algodão. |
Data corrente: |
14/03/2023 |
Data da última atualização: |
14/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MASSARIOLI, A. P.; SARTORI, A. G. de O.; JULIANO, F. F.; SANTOS, R. C. dos; RAMOS, J. P. C.; LIMA, L. M. de L.; ALENCAR, S. M. de. |
Afiliação: |
ADNA P. MASSARIOLI, USP - ESALQ; ALAN G. DE O. SARTORI, USP - ESALQ; FERNANDA F. JULIANO, USP - ESALQ; ROSEANE C. DOS SANTOS, CNPA; JEAN PIERRE C. RAMOS; LIZIANE MARIA DE LIMA, CNPA; SEVERINO MATIAS DE ALENCAR, USP - ESALQ. |
Título: |
Optimizing procedures for antioxidant phenolics extraction from skin and kernel of peanuts with contrasting levels of drought tolerance. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Foods, v. 11, n. 3, 449, p. 2-14, 2022. |
ISSN: |
2304-8158 |
DOI: |
https://doi.org/10.3390/foods11030449 |
Idioma: |
Inglês |
Conteúdo: |
Peanut is an affordable legume known for its nutritional value and phenolic content. The kernel and skin of 14 peanut genotypes contrasting in drought tolerance had their phenolic profiles determined and reactive oxygen species (ROS) scavenging activity evaluated. Firstly, temperature and % EtOH to extract antioxidant phenolic compounds were optimized using response surface methodology (RSM). The optimized extraction conditions, 60 ºC and 35% EtOH for kernels and 40 ºC and 60% EtOH for skins, were further adopted, and phenolic compounds were identified and quantified using high-performance liquid chromatography coupled with electrospray ionizationquadrupole- time of flight-mass spectrometry (HPLC-ESI-QTOF-MS) and high-performance liquid chromatography with photodiode array detector (HPLC-PDA). As a result, phenolic acids and glycosidic/non-glycosidic flavonoids were found. Principal component analysis was conducted, and the pairwise score plot of the skin extracts based on individual phenolic compounds showed a trend of genotype clustering based not only on drought tolerance but also on botanical type of germplasm. Therefore, our results demonstrate the status quo for antioxidant phenolic compounds of peanut genotypes contrasting in drought tolerance grown under natural field conditions. |
Palavras-Chave: |
Espécies reagentes ao oxigênio; Estresse hídrico; Flavonóides; Groundnut; Metodologia; Superfície de resposta. |
Thesagro: |
Amendoim. |
Thesaurus NAL: |
Flavonoids; Reactive oxygen species; Response surface methodology; Water stress. |
Categoria do assunto: |
Q Alimentos e Nutrição Humana |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1152353/1/OPTIMIZING-PROCEDURES-ANTIOXIDANT-9.pdf
|
Marc: |
LEADER 02395naa a2200349 a 4500 001 2152353 005 2023-03-14 008 2022 bl uuuu u00u1 u #d 022 $a2304-8158 024 7 $ahttps://doi.org/10.3390/foods11030449$2DOI 100 1 $aMASSARIOLI, A. P. 245 $aOptimizing procedures for antioxidant phenolics extraction from skin and kernel of peanuts with contrasting levels of drought tolerance.$h[electronic resource] 260 $c2022 520 $aPeanut is an affordable legume known for its nutritional value and phenolic content. The kernel and skin of 14 peanut genotypes contrasting in drought tolerance had their phenolic profiles determined and reactive oxygen species (ROS) scavenging activity evaluated. Firstly, temperature and % EtOH to extract antioxidant phenolic compounds were optimized using response surface methodology (RSM). The optimized extraction conditions, 60 ºC and 35% EtOH for kernels and 40 ºC and 60% EtOH for skins, were further adopted, and phenolic compounds were identified and quantified using high-performance liquid chromatography coupled with electrospray ionizationquadrupole- time of flight-mass spectrometry (HPLC-ESI-QTOF-MS) and high-performance liquid chromatography with photodiode array detector (HPLC-PDA). As a result, phenolic acids and glycosidic/non-glycosidic flavonoids were found. Principal component analysis was conducted, and the pairwise score plot of the skin extracts based on individual phenolic compounds showed a trend of genotype clustering based not only on drought tolerance but also on botanical type of germplasm. Therefore, our results demonstrate the status quo for antioxidant phenolic compounds of peanut genotypes contrasting in drought tolerance grown under natural field conditions. 650 $aFlavonoids 650 $aReactive oxygen species 650 $aResponse surface methodology 650 $aWater stress 650 $aAmendoim 653 $aEspécies reagentes ao oxigênio 653 $aEstresse hídrico 653 $aFlavonóides 653 $aGroundnut 653 $aMetodologia 653 $aSuperfície de resposta 700 1 $aSARTORI, A. G. de O. 700 1 $aJULIANO, F. F. 700 1 $aSANTOS, R. C. dos 700 1 $aRAMOS, J. P. C. 700 1 $aLIMA, L. M. de L. 700 1 $aALENCAR, S. M. de 773 $tFoods$gv. 11, n. 3, 449, p. 2-14, 2022.
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